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Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

Published on: November 25, 2009

Apertureless near-field Raman spectroscopy.

J J Wang¹, D A Smith, D N Batchelder

¹Department of Physics and Astronomy, University of Leeds, Leeds LS2 9JT, UK. phyjjw@phynov.leeds.ac.uk

Journal of Microscopy

|June 6, 2003

Summary

We demonstrate tip-enhanced Raman spectroscopy using a gold-coated atomic force microscope tip. This method allows simultaneous topographic and spectral analysis of C60 molecules, showcasing the tip enhancement effect.

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Area of Science:

Nanotechnology
Spectroscopy
Materials Science

Background:

Raman spectroscopy provides valuable molecular information but often suffers from weak signals.
Scanning probe microscopy offers high-resolution surface imaging and manipulation capabilities.

Purpose of the Study:

To investigate the application of tip-enhanced Raman spectroscopy (TERS) for analyzing C60 molecules.
To demonstrate the capability of a custom-built apertureless scanning near-field optical microscope (SNOM) for simultaneous topographic and spectral measurements.

Main Methods:

Utilized a custom-built apertureless SNOM system.
Employed a commercial atomic force microscope (AFM) tip coated with 100 nm of gold.
Acquired tip-enhanced Raman spectra of C60 samples.

Main Results:

Successfully obtained tip-enhanced Raman spectra of C60.
Demonstrated the localized enhancement of the Raman signal by the gold-coated AFM tip.
Achieved simultaneous acquisition of topographic and spectral data.

Conclusions:

The developed TERS system effectively enhances Raman signals for molecular analysis.
The gold-coated AFM tip plays a crucial role in the signal enhancement.
This technique shows promise for high-resolution chemical imaging of materials like C60.