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Successful transplantation of a 20% partial liver graft in rats: a technical innovation
Hideaki Tanaka1, Kohei Hashizume, Shin Enosawa
1Department of Innovative Surgery, National Research Institute for Child Health and Development, Tokyo 154-8567, Japan. htanaka-tky@umin.ac.jp
Background:
Graft-size disparity negatively impacts the postoperative outcome in the setting of adult-to-adult living donor liver transplantation.
Materials And Methods:
As a model for partial liver transplantation in rats using a minimized graft volume, we orthotopically transplanted a 20% reduced-size liver with the two-cuff method. Blood chemistry, graft weight restoration, and labeling indices of 5-bromo-2'-deoxyuridine were estimated.
Results:
We achieved 100% 2-week survival. Hepatic mass was restored rapidly with early normalization of biochemical parameters, although albumin synthesis capacity required more time to recover.
Conclusion:
This technique has great potential for research as well as a therapeutic approach to regeneration and functional recovery of small liver grafts under various posttransplant circumstances.