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Inactivated suckling mouse brain rabies vaccine provides short-term immunity in capuchin monkeys (Cebus apella)
E C Passos1, P M Germano, M A Guimarães
1Instituto Pasteur, Av. Paulista, 393, São Paulo, SP, Brazil 01311-000.
Abstract:
Eight capuchin monkeys (Cebus apella) were vaccinated against rabies with an inactivated suckling mouse brain vaccine (SMBV). Three 1-ml doses of 2% brain tissue suspension were given by i.m. injection at 0, 30, and 60 days. Blood samples were collected at 0, 30, 60, 90, 150, 210, 240, 300, and 365 days and were tested by simplified fluorescence inhibition to titer-neutralizing antibodies. All of the animals developed neutralizing antibodies with titers >0.5 IU/ml after vaccination, but the immune response persisted for only 122.3 +/- 32.6 days. The SMBV was able to induce immune response in the capuchin monkeys, but protection was short-lived.
Insights
This study shows that inactivated suckling mouse brain vaccine (SMBV) induced a rabies immune response in capuchin monkeys. However, the neutralizing antibody titers were short-lived, indicating limited long-term protection.
Area of Science:
- Veterinary Immunology
- Virology
- Primate Research
Background:
- Rabies remains a significant public health concern, necessitating effective vaccination strategies in various animal populations.
- Capuchin monkeys (Cebus apella) are relevant models for studying vaccine efficacy due to their physiological similarities to humans.
Purpose of the Study:
- To evaluate the immunogenicity and duration of the immune response to an inactivated suckling mouse brain vaccine (SMBV) against rabies in capuchin monkeys.
Main Methods:
- Eight capuchin monkeys received three intramuscular injections of 1-ml doses of 2% SMBV suspension at 0, 30, and 60 days.
- Serum samples were collected serially over 365 days and analyzed for rabies-neutralizing antibodies using a simplified fluorescence inhibition assay.
Main Results:
- All vaccinated capuchin monkeys developed neutralizing antibodies with titers exceeding 0.5 IU/ml post-vaccination.
- The induced immune response, measured by antibody titers, persisted for a mean duration of 122.3 +/- 32.6 days.
Conclusions:
- The inactivated suckling mouse brain vaccine (SMBV) is capable of eliciting an immune response in capuchin monkeys.
- The duration of protection conferred by this SMBV in capuchin monkeys is limited, suggesting a need for further investigation into vaccine strategies for sustained immunity.