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Related Experiment Videos

Microbial desizing using starch as model compound: enzyme properties and desizing efficiency.

Heiko Feitkenhauer1, Daniel Fischer, Daniel Fäh

  • 1Laboratory of Chemical Engineering and Industrial Chemistry, ETH-Zürich, Hönggerberg HCI, CH-8093 Zürich, Switzerland. feitkenhauer@tech.chem.ethz.ch

Biotechnology Progress
|June 7, 2003
PubMed
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Microbial desizing offers a sustainable alternative to conventional textile washing. This bioprocess utilizes acidifying cultures to partially degrade sizing agents, reducing effluent load and chemical use.

Area of Science:

  • Biotechnology
  • Textile Engineering
  • Environmental Science

Background:

  • Sizing agents protect yarn during weaving but their removal causes significant effluent load.
  • Conventional desizing requires substantial auxiliary chemicals, including surfactants.
  • Microbial desizing presents a novel bioprocess for eco-friendly sizing agent removal.

Purpose of the Study:

  • Characterize enzymatic properties of microbial desizing culture supernatant.
  • Link enzymatic activity to desizing efficiency.
  • Evaluate the bioprocess for starch-based sizing agents.

Main Methods:

  • Culturing acidifying microorganisms from anaerobic digestion plants.
  • Assessing enzymatic activity in culture supernatant using soluble starch.

Related Experiment Videos

  • Measuring metabolization rates of different starch fractions (amylose, dextrins, amylopectin).
  • Conducting desizing tests with dyed maize starch.

    • Main Results:

    • Optimal enzymatic activity observed between pH 4-5 and a broad temperature range (20-80°C).
    • Highest metabolization rates achieved with amylose; slower conversion of dextrins and amylopectin.
    • Enzymatic activity half-life of 45 hours at 37°C.
    • Significant starch chain length reduction within 1 hour, enabling solubilization.

    Conclusions:

    • The microbial desizing bioprocess effectively removes and partially degrades starch-based sizing agents.
    • The characterized enzymatic properties support its application in textile finishing.
    • This approach offers a sustainable reduction in effluent load and chemical consumption.