[Study on quality standard for heshouwu dispensing granule]

Area of Science:

• Herbal Medicine Quality Control
• Pharmacognosy
• Analytical Chemistry

Context:

• Heshouwu (Polygonum multiflorum) is a traditional Chinese medicine with a long history of use.
• Dispensing granules are a modern dosage form for traditional herbs, requiring stringent quality standards.
• Ensuring the quality of herbal medicines is crucial for patient safety and therapeutic efficacy.

Purpose:

• To establish and validate analytical methods for the quality control of Heshouwu dispensing granule.
• To identify Heshouwu using Thin Layer Chromatography (TLC).
• To quantify the key active compound, 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glucoside, using High-Performance Liquid Chromatography (HPLC).

Summary:

• Thin Layer Chromatography (TLC) was employed for the identification of Heshouwu dispensing granule.
• High-Performance Liquid Chromatography (HPLC) was utilized to determine the content of 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glucoside.
• The HPLC method demonstrated a linear relationship in the range of 0.4028-3.6252 micrograms, with an average recovery of 101.15% and a Relative Standard Deviation (RSD) of 2.00%.

Impact:

• The developed analytical methods are reproducible and suitable for routine quality control of Heshouwu dispensing granule.
• This study provides a reliable framework for ensuring the consistent quality and safety of Heshouwu dispensing granule.
• The validated methods contribute to the standardization of herbal medicines in pharmaceutical practice.