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[Establishment of human multidrug-resistant lung carcinoma cell line (D6/MVP)]
Sheng-lin Ma1, Jian-guo Feng, Lin-hui Gu
1Department of Medical Oncology, Zhejiang Cancer Hospital, Hangzhou 310022, China.
Objective:
To establish human multidrug-resistant lung carcinoma cell line (D6/MVP) with its characteristics studied.
Methods:
Intermittent administration of high-dose MMC, VDS and DDP (MVP) was used to induce human lung carcinoma cell line (D6) to a multidrug-resistant variety (D6/MVP). MTT assay was used to study the multidrug resistance of D6/MVP to multianticarcinogen. Flow cytometry was used to study the cell cycle distribution and the expression of P-gp, multidrug resistance-associated protein (MRP) and GSH/GST.
Results:
1. D6/MVP was resistant to many anti-tumor agents, with the IC(50) 13.3 times higher and the drug resistance 2 - 6 times higher than D6, 2. The multiplication time of D6/MVP was prolonged and the cell number of S-phase decreased while that of G1- and G(2)-phase increased and 3. The expression of P-gp and MRP was enhanced significantly (96.2% vs 51.7%), but the expression of GSH/GST kept stable.
Conclusion:
D6/MVP is a multidrug-resistant cell line possessing the basic characteristics of drug-resistance.
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