Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dyskinetoplastic Trypanosoma brucei contains functional editing complexes.

Gonzalo J Domingo1, Setareh S Palazzo, Bingbing Wang

  • 1Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.

Eukaryotic Cell
|June 11, 2003
PubMed
Summary

Kinetoplastid protozoa utilize RNA editing for functional mRNAs. Surprisingly, RNA editing complexes remain functional even without their natural substrates, pre-mRNA and guide RNA (gRNA).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Collective Oscillations of Photothermal Micromotors under Static Illumination.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Observation of localization reversal and harmonic generation in nonlinear non-Hermitian skin effect.

Nature communications·2026
Same author

Flow diverters for intracranial aneurysm embolization with coil embolization in children with ruptured aneurysm: two cases reports.

Frontiers in stroke·2026
Same author

Point of care performance of the improved BIOCREDIT Ag Pf/Pv (pLDH/pLDH) rapid diagnostic test: a cross-sectional study at a health center setting in Ethiopia.

Malaria journal·2026
Same author

Clinical perspectives on hemoglobin measurement: The example of malaria radical cure.

PLOS global public health·2026
Same author

Acoustic Features and Recognition of Distress Calls in <i>Rhinolophus nippon</i>: A Study Combining Machine Learning and Playback Experiments.

Biology·2026

Area of Science:

  • Molecular Biology
  • Genetics
  • Parasitology

Background:

  • Mitochondrial pre-mRNAs in kinetoplastids require posttranscriptional RNA editing, guided by small guide RNAs (gRNAs), to become functional mRNAs.
  • This editing process is catalyzed by a multienzyme protein complex and involves genetic material encoded in the maxicircle and minicircle components of kinetoplastid mitochondrial DNA (kDNA).

Purpose of the Study:

  • To investigate the necessity of pre-mRNA and gRNAs for the formation and function of RNA-editing complexes in kinetoplastids.
  • To determine if RNA-editing complexes retain their catalytic activities in the absence of their natural substrates.

Main Methods:

  • Analysis of Trypanosoma evansi AnTat3/3 (lacking maxicircles) and a dyskinetoplastic Trypanosoma brucei mutant (devoid of kDNA).
  • Immunoprecipitation and sedimentation assays to characterize the editing complexes.

Related Experiment Videos

  • In vitro assays to assess the catalytic activities of the isolated editing complexes.

    • Main Results:

    • Both T. evansi and T. brucei mutants retained genes and proteins essential for the RNA-editing complex.
    • These proteins formed complexes that immunoprecipitated and sedimented similarly to wild-type complexes.
    • The isolated complexes demonstrated catalytic activity in precleaved insertion/deletion and full-round deletion editing in vitro.

    Conclusions:

    • Functional RNA-editing complexes can form and retain catalytic activity independently of pre-mRNA and gRNAs.
    • Neither the pre-mRNA nor the gRNA is required for the assembly or function of these essential RNA-editing complexes in vitro.
    • This finding challenges the substrate-dependent model for RNA-editing complex formation in kinetoplastids.