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Half-genome human immunodeficiency virus type 1 constructs for rapid production of reporter viruses
Ayub Ali1, Beth D Jamieson, Otto O Yang
1Division of Infectious Diseases, Department of Medicine and AIDS Institute, Los Angeles Medical Center, University of California, 37-121 CHS, 10833 LeConte Avenue, Los Angeles, CA 90095-1688, USA.
Abstract:
The use of reporter constructs of HIV-1 molecular clones has been useful in studying the effects of infection on individual cells. A strategy of producing viral half-genome constructs containing reporter genes that can be used to produce recombinant complete infectious virions is described. This approach allows rapid generation of reporter and non-reporter virions from the same genetic construct, obviating the need to produce a new reporter genetic construct for each viral change to be studied. We provide an example of the utility of this system for studying the effects of Nef on infected cells.