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Updated: Sep 25, 2026
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Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
Published on: May 13, 2020
A low background high-throughput screening (HTS) fluorescence assay for lipases and esterases using
Emmanuel Leroy1, Nicolas Bensel, Jean Louis Reymond
1Department of Chemistry & Biochemistry, University of Bern, Freiestrasse 3, 3012, Bern, Switzerland.
Bioorganic & Medicinal Chemistry Letters
|June 12, 2003
Abstract:
Esters and acyloxymethyl ethers of umbelliferone were evaluated as fluorogenic substrates for lipases and esterases with respect to stability and resistance to non-specific hydrolysis. Isobutyryloxymethyl ether 1c and pivaloxymethyl ether 1d were found to be optimal substrates for enzyme assays, particularly with respect to HTS applications.
