Related Experiment Video
Updated: Aug 7, 2026
Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
Published on: November 14, 2016
Expression and cell-specific localization of cholecystokinin receptors in rat lung
Bin Cong1, Shu-Jin Li, Yi-Ling Ling
1Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China.
Aim:
To elucidate whether CCK receptors exist in lung tissues and their precise cellular localization in the lung.
Methods:
CCK-AR and CCK-BR mRNA expression and cellular distribution in the rat lung were detected by highly sensitive method of in situ reverse transcription-polymerase chain reaction (RT-PCR) and conventional in situ hybridization.
Results:
CCK-AR and CCK-BR gene positive signals were observed in bronchial epithelial cells, alveolar epithelial cells, pulmonary macrophages and vascular endothelial cells of the rats' lung by in situ RT-PCR. The hybridization signals of CCK-AR were relatively faint. By in situ hybridization, however, only the signals of CCK-BR but not CCK-AR were detected in the lung, and the positive staining was only found in vascular endothelial cells and macrophages.
Conclusion:
CCK-AR and CCK-BR gene were present in pulmonary vascular endothelial cells, macrophages, bronchial epithelial cells and alveolar epithelial cells, which play an important role in mediating the regulatory actions of CCK-8 on these cells.
