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Updated: Jul 24, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Structure of the polysaccharide chains of Pseudomonas pseudomallei lipopolysaccharides
Y A Knirel1, N A Paramonov, A S Shashkov
1N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
Abstract:
The pathogenic bacterium Pseudomonas pseudomallei strain 57576 produces two partially O-acetylated O-antigenic polysaccharides (PS-I and PS-II). Methylation analysis and 1H and 13C NMR spectroscopy, including NOE experiments, showed PS-I to have the structure [formula: see text] and PS-II to have the structure [formula: see text] where 6dmanHep is the unusual higher sugar 6-deoxy-D-manno-heptose. PS-II is produced also by P. pseudomallei strains 100 and 110, and PS-I and O-deacetylated PS-II by strain 97.
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