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Updated: Aug 1, 2026

Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos
Published on: August 3, 2017
Is cell surface calreticulin involved in phagocytosis by insect hemocytes?
1Department of Applied and Molecular Ecology, The University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia. sassan.asgari@adelaide.edu.au
Abstract:
The innate immune system of insects consists of humoral and cellular components involved in the recognition of and responses to intruding foreign micro- or macroorganisms. Several molecules have been identified so far that recognize molecular patterns present on microorganisms, such as lipopolysaccharides, peptidoglycans and lipoteichonic acid. These molecules, acting as opsonins, trigger immune responses such as phagocytosis, nodule formation, melanization and encapsulation. Here, we investigated the role of calreticulin (CRT) present on the surface of Pieris rapae hemocytes in phagocytosis. Comparative phagocytosis assays using yeast cells showed that hemocytes from different insects exhibit significant variation in their phagocytosing potential and relative CRT involvement.
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