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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Published on: April 14, 2015

[Real time PCR methodology for quantification of nucleic acids]

C Tse1, J Capeau

  • 1Hôpital Tenon, Laboratoire de biochimie et d'hormonologie, 4, rue de la Chine, 75020 Paris.

Annales De Biologie Clinique
|June 14, 2003
PubMed
Summary

Real-time quantitative PCR (qPCR) revolutionizes DNA and RNA analysis by enabling precise quantification. This review covers qPCR principles, novel closed-tube assays like SYBR Green and TaqMan probes, and clinical applications.

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Overview
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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...