Related Experiment Video
Updated: Jul 24, 2026

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
Published on: January 22, 2018
An innovative approach for locating and evaluating subsurface pathways for nitrogen loss.
C L Walthall1, T J Gish, C S Daughtry
1USDA-ARS Hydrology and Remote Sensing Lab, Beltsville, MD 20705, USA. cwalthall@hydrolab.arsusda.gov
Understanding watershed chemical flux is crucial for mitigating contamination. New methods using ground-penetrating radar (GPR) and GIS effectively map subsurface flow pathways, aiding environmental protection strategies.
Area of Science:
- Environmental Science
- Hydrology
- Soil Science
Background:
- Watershed-scale chemical flux to ecosystems is poorly understood, hindering effective contamination mitigation strategies.
- Characterizing processes like evapotranspiration, runoff, plant uptake, and subsurface flow is essential.
- Conventional sampling methods fail to adequately measure preferential flow of water and solutes in subsurface environments.
Purpose of the Study:
- To develop and test a novel sampling strategy for accurately identifying subsurface preferential flow pathways.
- To understand how agricultural watershed management practices influence chemical transport.
- To characterize chemical flux from agricultural watersheds to adjacent ecosystems.
Main Methods:
- Utilized ground-penetrating radar (GPR) for subsurface structure mapping.
- Integrated near real-time soil moisture data, surface topography, and remotely sensed imagery.
- Employed a geographic information system (GIS) to analyze spatial data and identify flow pathways.
- Confirmed identified pathways using yield monitor data and remote sensing imagery.
Main Results:
- Successfully identified spatial locations of preferential flow pathways for chemicals exiting agricultural watersheds.
- Demonstrated the efficacy of the integrated GPR, GIS, and remote sensing approach for characterizing subsurface flow.
- Provided initial insights into the movement of chemicals from agricultural lands to riparian and stream ecosystems.
Conclusions:
- The developed sampling strategy effectively identifies subsurface preferential flow pathways, crucial for understanding chemical transport.
- This approach offers a significant advancement over conventional methods for characterizing complex subsurface hydrology.
- Accurate identification of these pathways is vital for formulating effective strategies to mitigate chemical contamination in downstream ecosystems.
Related Concept Videos
The Nitrogen Cycle
Key Elements for Plant Nutrition
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this nitrogen...
Inorganic Nitrogen Assimilation
Microbes and the Nitrogen Cycle
Microbial Leaching

