Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dendritic cells in glomerulonephritis.

S Cuzic1, E Ritz, R Waldherr

  • 1Department of Pathology, University of Heidelberg, Federal Republic of Germany.

Virchows Archiv. B, Cell Pathology Including Molecular Pathology
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

De novo glomerulonephritis in patients during remission from Wegener's granulomatosis.

Clinical nephrology·1992
Same author

Reduced capillary density in the myocardium of uremic rats--a stereological study.

Kidney international·1992
Same author

Renal histology in polycystic kidney disease with incipient and advanced renal failure.

Kidney international·1992
Same author

The effect of uninephrectomy on progression of renal failure in autosomal dominant polycystic kidney disease.

Journal of the American Society of Nephrology : JASN·1992
Same author

Severe hemorrhagic complications from infection with nephropathia epidemica strain of Hantavirus.

Clinical nephrology·1992
Same author

Disturbed calcium metabolism in renal failure--pathogenesis and therapeutic strategies.

Kidney international. Supplement·1992
Same journal

Peroxisomes in liver, kidney and duodenum of nude mice bearing xenografts of human pancreatic adenocarcinomas.

Virchows Archiv. B, Cell pathology including molecular pathology·1993
Same journal

Ultrastructural study of hepatic granulomas induced by Cryptococcus neoformans by quick-freezing and deep-etching method.

Virchows Archiv. B, Cell pathology including molecular pathology·1993
Same journal

Inhibition of the effects of rheumatoid synovial fluid cells on chondrogenesis and cartilage breakdown in vitro: possible therapeutical conclusions. A morphological--biochemical study.

Virchows Archiv. B, Cell pathology including molecular pathology·1993
Same journal

Gastrointestinal AAPOAII and systemic AA-amyloidosis in aged C57BL/Ka mice. Amyloid-type dependent effect of long-term immunosuppressive treatment.

Virchows Archiv. B, Cell pathology including molecular pathology·1993
Same journal

The effects of obstruction and secretory stimulation on microlithiasis in salivary glands of cat: light and electron microscopy.

Virchows Archiv. B, Cell pathology including molecular pathology·1993
Same journal

Smooth muscle differentiation in human myometrium and uterine leiomyoma.

Virchows Archiv. B, Cell pathology including molecular pathology·1993
See all related articles

Dendritic cells (DCs) expressing CD1b are involved in interstitial inflammation in glomerulonephritis (GN). Their presence correlates with de novo HLA-DR/Ii expression in tubular cells, suggesting a role in kidney disease progression.

Area of Science:

  • Nephrology
  • Immunology
  • Pathology

Background:

  • Glomerulonephritis (GN) encompasses various kidney diseases characterized by inflammation of the glomeruli.
  • Dendritic cells (DCs) are key immune cells involved in initiating and regulating immune responses.
  • The role of DCs in the pathogenesis of different forms of GN, particularly in relation to interstitial inflammation and tubular changes, requires further elucidation.

Purpose of the Study:

  • To investigate the presence and distribution of CD1b-positive dendritic cells (DCs) in renal biopsies from patients with various forms of glomerulonephritis (GN).
  • To examine the relationship between DCs and interstitial inflammation, glomerular pathology, and de novo expression of HLA-DR and invariant chain (Ii) in proximal tubular epithelial cells in GN.
  • To elucidate the potential role of DCs in the development of interstitial inflammation and tubular cell alterations in GN.

Related Experiment Videos

Main Methods:

  • Analysis of 45 renal biopsies from patients with mesangial IgA-GN, focal glomerular sclerosis, and acute GN, alongside 7 normal kidney controls.
  • Double staining immunocytochemistry (APAAP, streptavidin-peroxidase) was employed using monoclonal antibodies against CD1b (DCs), invariant chain (Ii), and HLA-DR.
  • Assessment of the co-localization of CD1b-positive DCs with inflammatory cells and their proximity to affected glomeruli, as well as de novo HLA-DR/Ii expression in tubular epithelial cells.

Main Results:

  • CD1b-positive DCs were absent in normal kidneys and in GN cases without interstitial inflammation.
  • In GN biopsies with interstitial inflammation (n=36), CD1b-positive DCs were identified among inflammatory cells.
  • In IgA-GN, DCs were found near glomeruli with crescents or sclerosis. De novo HLA-DR/Ii expression in tubular cells was observed only in the presence of DCs.

Conclusions:

  • CD1b-positive DCs are significantly associated with interstitial inflammation in various forms of glomerulonephritis.
  • The presence of DCs may contribute to the de novo coexpression of HLA-DR and invariant chain in proximal tubular epithelial cells, potentially via cytokine production.
  • These findings highlight the important role of dendritic cells in the inflammatory processes and tubular pathology observed in glomerulonephritis.