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Activity staining of endoglucanases in polyacrylamide gels
1Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda, India.
Analytical Biochemistry
|October 1, 1992
Abstract:
The endoglucanases of Penicillium funiculosum were analyzed for the presence of multiple forms using a modified version of the Congo red method. Postelectrophoretic slab gels were directly incubated in a solution of carboxymethylcellulose for a period as short as 15 min and then the activities were visualized by staining with Congo red. Ten distinct bands of clearances were obtained indicating the presence of at least as many multiple forms.