Determinants of substrate recognition in nonreceptor tyrosine kinases

Cytoplasmic tyrosine kinases do not occur as isolated catalytic domains. Instead, each kinase family possesses a characteristic array of additional domains that are appended to the catalytic domain. The combination and the arrangement of these modular domains are important in kinase regulation and function. This Account describes how the noncatalytic regions of Src family tyrosine kinases are involved in enzyme regulation, substrate selection, and multisite phosphorylation.