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Updated: May 4, 2026

Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
Chemical approaches to reversible protein phosphorylation
Philip A Cole1, Aliya D Courtney, Kui Shen
1Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA. pcole@jhmi.edu
Abstract:
Protein phosphorylation catalyzed by protein kinases plays a critical role in cellular signaling. Here we review several chemical approaches to understanding protein kinases and the consequences of protein phosphorylation. We discuss the design of bisubstrate analogue inhibitors based on a dissociative transition state, the development of reagents for cross-linking protein kinases with their substrates, the chemical rescue of mutant protein tyrosine kinases, and the application of expressed protein ligation to understanding protein phosphorylation.
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