Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diagnostic techniques.

J H Brinser1

  • 1Department of Ophthalmology, College of Medicine, University of South Florida, Tampa.

Infectious Disease Clinics of North America
|December 1, 1992
PubMed
Summary
This summary is machine-generated.

Proper ocular microbiology techniques minimize confusion from normal flora in eye cultures. Quantifying microbial growth aids in determining the significance of isolates from various ocular specimens.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute conjunctivitis in childhood.

The Journal of pediatrics·1993
Same author

Acridine orange and Gram stains in infectious keratitis.

Cornea·1990
Same author

Exogenous bacterial endophthalmitis treated without systemic antibiotics.

American journal of ophthalmology·1987
Same author

Haemophilus aphrophilus as a cause of crystalline keratopathy.

American journal of ophthalmology·1987
Same author

Treatment of experimental methicillin-resistant Staphylococcus epidermidis endophthalmitis with intravitreal vancomycin.

Ophthalmology·1987
Same author

Exfoliated cytopathology of Acanthamoeba keratitis.

JAMA·1986
Same journal

Prevention and Control of Clostridioides difficile Infection for the Infectious Diseases Clinician.

Infectious disease clinics of North America·2026
Same journal

Infection Control Strategies to Prevent Emergence and Transmission of Resistant Gram-Negative Bacteria.

Infectious disease clinics of North America·2026
Same journal

Preventing the Spread of Tuberculosis in Health Care Settings.

Infectious disease clinics of North America·2026
Same journal

Threats to Success: Principles of Infection Prevention and Control in Health Care Settings, Part 2: Device and Pathogen Management.

Infectious disease clinics of North America·2026
Same journal

Ventilator-Associated Events: Surveillance and Prevention.

Infectious disease clinics of North America·2026
Same journal

Nosocomial Fungal Infections: Epidemiology, Control Strategies, and Prevention of Candida and Other Yeasts.

Infectious disease clinics of North America·2026
See all related articles

Area of Science:

  • Ophthalmology
  • Microbiology
  • Clinical Diagnostics

Background:

  • Ocular infections present diagnostic challenges due to common environmental and normal flora contaminants.
  • Distinguishing pathogenic from non-pathogenic microorganisms in ocular specimens is crucial for effective treatment.

Purpose of the Study:

  • To describe and validate ocular microbiology techniques that reduce contamination and improve isolate interpretation.
  • To enable quantitative assessment of microbial growth from ocular samples.

Main Methods:

  • Detailed description of specimen inoculation techniques for ocular microbiology.
  • Culturing methods for lid, conjunctiva, cornea, anterior chamber, and vitreous fluid specimens.
  • Quantitative analysis of microbial growth.

Related Experiment Videos

Main Results:

  • Implemented techniques significantly reduce confusion caused by normal flora and environmental organisms.
  • Quantitative data allows for comparison between different ocular sites (e.g., lid vs. cornea).
  • Comparison of growth patterns provides valuable clues to the clinical significance of identified microorganisms.

Conclusions:

  • Standardized ocular microbiology techniques enhance diagnostic accuracy.
  • Quantitative culturing aids in differentiating true pathogens from contaminants in eye infections.
  • These methods improve the interpretation of microbial isolates from ocular specimens.