Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mgr, a novel global regulator in Staphylococcus aureus.

Thanh T Luong1, Steven W Newell, Chia Y Lee

  • 1Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA.

Journal of Bacteriology
|June 19, 2003
PubMed
Summary

A novel gene, mgrA, regulates Staphylococcus aureus virulence factors. It activates capsule and nuclease but represses toxins and proteases, impacting bacterial infection strategies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of Ssc, an <i>N</i>-acetylgalactosamine-containing <i>Staphylococcus aureus</i> surface polysaccharide.

Journal of bacteriology·2024
Same author

Deconstructing the Excellent Plastic Surgeon: A Survey of Key Attributes.

Plastic and reconstructive surgery. Global open·2023
Same author

A SWOT Analysis of Hot Topics in Plastic Surgery Resident Education: Consensus From the ACAPS 10th Annual Winter Meeting.

Plastic and reconstructive surgery. Global open·2023
Same author

Clinical Impact and Safety of Non-Target Punctures (NTP) during Portal Vein Access in TIPS Procedure.

Biomedicines·2023
Same author

Regulation of Staphylococcal Capsule by SarZ is SigA-Dependent.

Journal of bacteriology·2022
Same author

A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm Proteins.

Methods in molecular biology (Clifton, N.J.)·2021

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Pathogenesis

Background:

  • Staphylococcus aureus virulence is controlled by multiple chromosomal loci.
  • Understanding these regulatory networks is crucial for combating bacterial infections.

Purpose of the Study:

  • To identify and characterize a novel genetic locus regulating virulence factor expression in Staphylococcus aureus.
  • To elucidate the function of the identified gene, mgrA, in controlling key virulence determinants.

Main Methods:

  • Genetic mapping using transposon mutagenesis and three-factorial transduction crosses.
  • Cloning, sequencing, deletion mutagenesis, and genetic complementation of the mgr locus.
  • Analysis of gene expression using Northern slot blot and assessment of extracellular protein production via SDS-PAGE.

Related Experiment Videos

Main Results:

  • Identified a novel locus, mgr, containing a single gene, mgrA.
  • A mutation upstream of mgrA in strain CYL5614 led to increased mgrA transcription (4-8 fold).
  • mgrA acts as an activator for type 8 capsular polysaccharide (CP8) and nuclease, but a repressor for alpha-toxin, coagulase, protease, and protein A.
  • The MgrA protein shares homology with the MarR family of transcriptional regulators, including a helix-turn-helix DNA-binding motif.
  • The mgr locus significantly impacts extracellular protein production and affects gene expression at the transcriptional level.

Conclusions:

  • mgrA is a key transcriptional regulator controlling a wide range of Staphylococcus aureus virulence factors.
  • The dual role of mgrA as both an activator and repressor highlights its complex role in coordinating bacterial pathogenesis.
  • mgrA represents a potential target for therapeutic intervention against Staphylococcus aureus infections.