Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Novel light microscopy imaging techniques in nephrology.

Robert L Bacallao1, Weiming Yu, Kenneth W Dunn

  • 1Division of Nephrology, Richard Roudebush VAMC and Indiana University School of Medicine, Indianapolis, Indiana, USA.

Current Opinion in Nephrology and Hypertension
|June 20, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robotic-assisted versus laparoscopic esophageal hiatal hernia and anti-reflux surgery: A comprehensive systematic review and meta-analysis.

Hernia : the journal of hernias and abdominal wall surgery·2026
Same author

Neural-tumor crosstalk in driving tumor metabolic reprogramming.

Biochimica et biophysica acta. Reviews on cancer·2026
Same author

CD4<sup>+</sup> T cells impair tumor growth through IL-3 and TNF-dependent vascular damage.

Science (New York, N.Y.)·2026
Same author

Hierarchical integration of carbon dot on Ce-MOF nanozymes in a hydrogel for diabetic alveolar bone repair.

Biomaterials advances·2026
Same author

Artificial intelligence facilitates urban green transition in the Yangtze River Delta urban agglomeration.

Scientific reports·2026
Same author

Microfluidic chips for decoding cancer-immune crosstalk in immunotherapy.

Frontiers in immunology·2026
Same journal

Updates in management of cardiorenal syndrome.

Current opinion in nephrology and hypertension·2026
Same journal

Sex differences in renal acid-base regulation.

Current opinion in nephrology and hypertension·2026
Same journal

Primary prevention of chronic kidney disease in type 2 diabetes mellitus with sodium-glucose cotransporter 2 inhibitors.

Current opinion in nephrology and hypertension·2026
Same journal

Financial and policy challenges of delivering kidney replacement therapies in resource-limited settings.

Current opinion in nephrology and hypertension·2026
Same journal

The role of kir4.1/Kir5.1 in mediating the effect of angiotensin-II on Na-Cl-cotransporter.

Current opinion in nephrology and hypertension·2026
Same journal

Role of the calcium-sensing receptor in regulating calcium transport in the thick ascending limb.

Current opinion in nephrology and hypertension·2026
See all related articles

New microscopy imaging techniques allow scientists to visualize gene products and cell function in vivo. These advanced optical methods, including two-photon confocal microscopy, are revolutionizing the study of whole organ physiology and renal function.

Area of Science:

  • Genomics and Proteomics
  • Physiology
  • Biotechnology

Background:

  • Genome sequencing generates vast amounts of data, necessitating efficient methods for characterizing gene products.
  • Advancements in transgenic animal models, gene transfer, and RNA silencing are crucial for understanding biological systems.
  • Emerging microscopy imaging technologies offer unprecedented capabilities for visualizing biological processes.

Purpose of the Study:

  • To review novel microscopy imaging techniques for characterizing gene products and cellular functions.
  • To discuss the advantages, imaging properties, and limitations of these advanced optical methods.
  • To highlight the application of these techniques in studying whole organ physiology and transgenic animal models.

Main Methods:

Related Experiment Videos

  • Two-photon confocal microscopy for in vivo imaging of organs like the kidney.
  • Fluorescence resonance energy transfer (FRET) for visualizing molecular interactions within cells, such as transcription factor complexes.
  • Total internal fluorescence reflectance microscopy (TIRFM) for observing protein localization at the plasma membrane.

    • Main Results:

    • Two-photon microscopy enables direct imaging within living animal kidneys.
    • FRET successfully visualizes the formation of transcription factor complexes in the nucleus.
    • TIRFM allows direct observation of protein trafficking to the cell surface.
    • These methods extend the understanding of whole organ and renal epithelial function.

    Conclusions:

    • Advanced optical techniques, coupled with fluorescent labeling, facilitate in vivo studies of cellular processes and organ function.
    • Light microscopy is transitioning from semi-quantitative to quantitative approaches in physiological research.
    • These innovations are vital for advancing research in renal function and other physiological areas.