Related Experiment Videos
[Cell membrane electroporator with digital generation of random shaped pulses]
1Physical Department, Lomonosov Moscow State University, Vorob'evy Gory, Moscow, 117234 Russia.
Biofizika
|June 21, 2003
Summary
A novel Digital Poration System (DPS) offers programmable electric field pulses for advanced biological treatments. This versatile electroporator overcomes limitations of existing devices, enabling wider applications in cell biology and biotechnology.
Area of Science:
- Biophysics
- Biotechnology
- Electrical Engineering
Context:
- Electroporation is a key technique for modifying cell membranes.
- Existing electroporation devices have limitations in pulse programmability and functionality.
- Capacitor-discharge electroporators lack precision and versatility.
Purpose:
- To design and implement a Digital Poration System (DPS) for versatile electrotreatment of biological objects.
- To overcome the drawbacks of conventional capacitor-discharge electroporators.
- To expand the application range of electroporation technology.
Summary:
- The Digital Poration System (DPS) utilizes a digital-to-analog converter for arbitrary pulse generation, including bipolar waveforms.
- It offers programmable pulse shapes, high slew rates (1 V/ns), and +/- 250 V output, surpassing traditional devices.
- DPS eliminates the need for external analyzers, recharging, and multiple auxiliary generators, simplifying operation.
Impact:
- Enables precise electrotreatment of biological membranes with customizable electric field pulses.
- Extends applications to electrochemotherapy, cell electrofusion, oocyte activation, cell sorting, and dielectrophoresis.
- Facilitates advanced research in cloning technology and cellular manipulation.