Characterization of the permselective properties of rabbit skin during transdermal iontophoresis

The use of iontophoresis to enhance transdermal transport of drugs necessitates further investigations on the membrane used to simulate human skin because one of the transport mechanisms involved, electroosmosis, strictly depends on the properties of the skin (i.e., its isoelectric point, IP). The aim of this work was to characterize rabbit ear skin permselectivity by measuring the sodium transport number at different pH values. This method allowed us to estimate the skin IP. To confirm the validity of the method, mannitol flux was measured. In addition, the sodium transport number method was applied to the study of human skin and a model drug iontophoretic flux through rabbit and human skin was evaluated. The results indicate that rabbit ear skin behaves as a permselective membrane, with an IP between 2 and 3. The same result was obtained using human skin. The mannitol flux data confirm that the direction of electroosmotic flow at physiological pH is in the anode-to-cathode direction. Finally, permeation experiments performed with a model drug showed that the relative electroosmotic and electrorepulsive contributions to the total flux are the same for human and rabbit skin. It can be concluded that rabbit ear skin is a suitable model for the study of iontophoretic permeation of drugs.