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Related Experiment Videos

A candidate olfactory receptor subtype highly conserved across different insect orders.

J Krieger1, O Klink, C Mohl

  • 1Institute of Physiology (230), University of Hohenheim, Garbenstr. 30, 70599 Stuttgart, Germany. krieger@uni-hohenheim.de

Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology
|June 27, 2003
PubMed
Summary

A unique olfactory receptor (HR2) in moths shows high conservation across insect orders. This suggests a crucial, specialized role in insect chemosensation, unlike other diverse insect olfactory receptors.

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Area of Science:

  • Insect Olfaction
  • Molecular Evolution
  • Chemosensory Neuroscience

Background:

  • Olfactory receptors (ORs) are crucial for insect chemosensation.
  • Insect ORs exhibit significant diversity across species, complicating comparative studies.
  • A specific OR, HR2 in Heliothis virescens, shows unusual conservation.

Purpose of the Study:

  • To identify and characterize HR2 homologues in other insect species.
  • To investigate the evolutionary conservation and expression patterns of HR2-related receptors.
  • To understand the potential specialized function of this conserved receptor subtype.

Main Methods:

  • Sequence analysis of candidate olfactory receptors.
  • RT-PCR (Reverse Transcription Polymerase Chain Reaction) to detect gene expression.

Related Experiment Videos

  • In situ hybridization to determine receptor localization in antennal neurons.

    • Main Results:

    • HR2 homologues were identified in two additional moth species (Antheraea pernyi, Bombyx mori) with high amino acid identity (86-88%).
    • HR2-related receptors were detected in diverse insect orders: Hymenoptera (Apis mellifera), Diptera (Calliphora erythrocephala), and Coleoptera (Tenebrio molitor).
    • In situ hybridization confirmed widespread expression of HR2-related receptors in numerous antennal neurons across species.

    Conclusions:

    • The HR2 receptor subtype is highly conserved across a broad range of insect orders.
    • Widespread expression in antennal neurons suggests a fundamental role in insect chemosensation.
    • This conserved receptor likely fulfills a specialized function in insect sensory perception.