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Localization of oncostatin M receptor beta in adult and developing CNS
S Tamura1, Y Morikawa, E Senba
1Department of Anatomy and Neurobiology, Wakayama Medical University, 811-1 Kimiidera, 641-8509, Wakayama, Japan.
Abstract:
Oncostatin M (OSM) is a member of the interleukin-6 cytokine family, which is involved in definitive hematopoiesis, the development of liver, and local inflammation. However, little is known about the role of OSM in the murine CNS. Using Northern blot analysis, we examined the regional distribution of OSM receptor beta (OSMRbeta) mRNA in the adult CNS. OSMRbeta mRNA was observed predominantly in the olfactory bulb, and with low levels in the other regions. In situ hybridization shows that OSMRbeta gene expression was found in astrocytes of olfactory bulb, epithelial cells of choroid plexus, and meningeal cells in pia mater. In addition, we investigated the gene expression of OSMRbeta in the developing CNS at different time points. Its gene expression was first observed in large neurons of the hypoglossal nucleus at 14.5 days postcoitum, which was sustained until neonatal mice. OSMRbeta mRNA and protein were mainly localized in the ventral subnucleus of the developing hypoglossal nucleus. Our results suggest that OSM contributes to the development of specific subpopulations of both neurons and astrocytes in the murine CNS.
Insights
Oncostatin M receptor beta (OSMRbeta) is found in specific brain regions like the olfactory bulb in adult mice. Its expression in the developing central nervous system (CNS) suggests a role in neuron and astrocyte development.
Area of Science:
- Neuroscience
- Molecular Biology
- Developmental Biology
Background:
- Oncostatin M (OSM) is an interleukin-6 family cytokine implicated in hematopoiesis, liver development, and inflammation.
- The precise role of OSM within the murine central nervous system (CNS) remains largely uncharacterized.
Purpose of the Study:
- To investigate the distribution and expression patterns of the OSM receptor beta (OSMRbeta) in the adult and developing murine CNS.
- To elucidate the potential role of OSM signaling in CNS development.
Main Methods:
- Northern blot analysis to determine regional mRNA distribution of OSMRbeta in the adult CNS.
- In situ hybridization to localize OSMRbeta gene expression in specific cell types and brain regions.
- Analysis of OSMRbeta expression during different developmental stages of the murine CNS.
Main Results:
- OSMRbeta mRNA was predominantly detected in the olfactory bulb of adult mice, with lower levels in other regions.
- Gene expression of OSMRbeta was identified in astrocytes of the olfactory bulb, choroid plexus epithelial cells, and meningeal cells.
- During development, OSMRbeta expression was observed in large neurons of the hypoglossal nucleus starting at 14.5 days postcoitum and persisted into the neonatal period.
- OSMRbeta mRNA and protein were primarily localized to the ventral subnucleus of the developing hypoglossal nucleus.
Conclusions:
- OSMRbeta exhibits a distinct regional distribution in the adult murine CNS, with notable expression in the olfactory bulb.
- OSM signaling is likely involved in the development of specific neuronal populations, particularly within the hypoglossal nucleus.
- The findings suggest a role for OSM in the development of both neurons and astrocytes in the murine CNS.