Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

HIV-1 reverse transcriptase inhibitor from Phyllanthus niruri.

T Ogata1, H Higuchi, S Mochida

  • 1Research Institute for Molecular Genetics, Tsumura & Co., Ibaraki-Ken, Japan.

AIDS Research and Human Retroviruses
|November 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predictors of ischemic bowel in patients with incarcerated hernias.

Hernia : the journal of hernias and abdominal wall surgery·2019
Same author

Lobeline production by hairy root culture of Lobelia inflata L.

Plant cell reports·2013
Same author

Surfactant therapy in patients with acute respiratory failure: report of two cases.

Journal of anesthesia·2013
Same author

The fourth step of protein synthesis: disassembly of the posttermination complex is catalyzed by elongation factor G and ribosome recycling factor, a near-perfect mimic of tRNA.

Cold Spring Harbor symposia on quantitative biology·2003
Same author

Inhibitory mechanisms of tea polyphenols on the ultraviolet B-activated phosphatidylinositol 3-kinase-dependent pathway.

The Journal of biological chemistry·2001
Same author

Suppression of cell transformation and induction of apoptosis by caffeic acid phenethyl ester.

Molecular carcinogenesis·2001
Same journal

Identification of a Complex HIV-1 Circulating Recombinant Form (CRF203_cpx) Originating from Three Main Circulating Recombinant Forms in China.

AIDS research and human retroviruses·2026
Same journal

Near Full-Length Genome Characterization of a Novel Second-Generation HIV-1 CRF01_AE/CRF07_BC Recombinant Identified in an MSM Individual in Guizhou, China.

AIDS research and human retroviruses·2026
Same journal

Evolving Indications and Diagnostic Spectrum of Bone Marrow Examination in People with HIV During the Antiretroviral Therapy Era: A 6-Year Retrospective Study.

AIDS research and human retroviruses·2026
Same journal

A Conserved Natural Killer Cell Transcriptional Fingerprint in Rhesus Macaque Models of Acute Viral Infections.

AIDS research and human retroviruses·2026
Same journal

Perceived Ethical Risks of HIV Phylogenetic Research: Insights from Professional Stakeholders in Uganda.

AIDS research and human retroviruses·2026
Same journal

Genomic Characterization of Two Novel Human Immunodeficiency Virus-1 Recombinant Forms (CRF01_AE and B) in Men Who Have Sex with Men in Hebei, China.

AIDS research and human retroviruses·2026
See all related articles

Repandusinic acid A (RA), isolated from Phyllanthus niruri, effectively inhibits human immunodeficiency virus type-1 reverse transcriptase (HIV-1-RT). This compound shows significant potential in combating HIV-1 replication and associated cellular damage.

Area of Science:

  • Phytochemistry
  • Virology
  • Molecular Biology

Background:

  • Phyllanthus niruri extract demonstrates inhibitory activity against HIV-1 reverse transcriptase (HIV-1-RT).
  • Identifying and characterizing novel antiviral compounds from natural sources is crucial for developing new therapeutic strategies against HIV-1.

Purpose of the Study:

  • To isolate and identify the specific compound in Phyllanthus niruri responsible for inhibiting HIV-1-RT.
  • To evaluate the antiviral efficacy and mechanism of action of the identified compound against HIV-1.

Main Methods:

  • Aqueous extract purification using Sephadex LH-20, cellulose, and reverse-phase HPLC.
  • Structure elucidation of the inhibitor via Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Enzyme inhibition assays (HIV-1-RT and DNA polymerase alpha) and cell-based assays (cytopathogenicity, giant cell formation, p24 antigen production).

Related Experiment Videos

Main Results:

  • Repandusinic acid A monosodium salt (RA), originally from Mallotus repandus, was identified as the active inhibitor.
  • RA exhibited a 10-fold higher sensitivity towards HIV-1-RT (ID50 = 0.05 microM) compared to DNA polymerase alpha (ID50 = 0.6 microM).
  • RA demonstrated competitive inhibition against template-primer and noncompetitive inhibition against the substrate, significantly reducing HIV-1-induced cytopathogenicity, giant cell formation, and p24 antigen production in various cell systems.

Conclusions:

  • Repandusinic acid A is a potent inhibitor of HIV-1-RT with significant antiviral activity.
  • RA's mechanism involves direct inhibition of viral reverse transcriptase and interference with viral replication processes.
  • Phyllanthus niruri and its active compound RA represent a promising avenue for developing novel anti-HIV therapies.