Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Novel cell junctions induced by activating Thy-1-specific antibodies.

M M Millrain1, R Hederer, S Griffiths

  • 1Clinical Research Centre, Harrow, Middlesex, UK.

International Immunology
|October 1, 1992
PubMed
Summary

Anti-Thy-1 antibodies, including KT16, cause T cell aggregation by forming specific junctions. This Thy-1 specific cell adhesion is linked to the antibody

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Involvement of brown adipose tissue in subcutaneous fat necrosis of the newborn.

Dermatology (Basel, Switzerland)·2011
Same author

Tc99m-hepatobiliary iminodiacetic acid (HIDA) scintigraphy in clinical practice.

Clinical radiology·2011
Same author

Fear of death in a sample of physicians.

Canadian family physician Medecin de famille canadien·2011
Same author

Hilar cholangiocarcinoma: MR correlation with surgical and histological findings.

Clinical radiology·2010
Same author

Effect of slice thickness on liver lesion detection and characterisation by multidetector CT.

Journal of medical imaging and radiation oncology·2010
Same author

Two fractions enriched for striatal synaptosomes isolated by percoll gradient centrifugation: synaptosome morphology, dopamine and serotonin receptor distribution, and adenylate cyclase activity.

Neurochemistry international·2010

Area of Science:

  • Immunology
  • Cell Biology
  • T cell activation

Background:

  • Anti-Thy-1 antibodies are known to induce T cell aggregation.
  • The precise mechanism and specificity of this aggregation are not fully understood.

Purpose of the Study:

  • To investigate the mechanism of T cell aggregation induced by anti-Thy-1 antibodies, specifically KT16.
  • To determine the specificity of the induced cell junctions and their relationship to antibody properties.

Main Methods:

  • Utilized Protein A-gold labeling to visualize antibody distribution on T cells.
  • Employed electron microscopy to examine cell junctions in KT16-treated T cells.
  • Compared aggregation induced by KT16 with that caused by concanavalin A and anti-CD3 antibodies.

Related Experiment Videos

Main Results:

  • KT16 and other anti-Thy-1 antibodies concentrate at intercellular contacts, forming specific cell junctions.
  • These junctions are Thy-1 specific and dependent on antibodies targeting particular epitopes.
  • The observed aggregation is distinct from that induced by concanavalin A or anti-CD3.
  • The extent of T cell adhesiveness correlates with the mitogenic capacity of anti-Thy-1 antibodies.

Conclusions:

  • Anti-Thy-1 antibodies induce T cell aggregation through a specific, Thy-1-dependent junctional mechanism.
  • This mechanism is distinct from other T cell aggregation pathways and is influenced by antibody epitope specificity.
  • T cell adhesiveness and mitogenic capacity are directly related for anti-Thy-1 antibodies.