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Authentic and recombinant bilirubin oxidases are in different resting forms
Takeshi Sakurai1, Lei Zhan, Takahiro Fujita
1Department of Chemistry, Faculty of Science, Kanazawa University, Kakuma, Kanazawa 920-1192, Japan. ts0513@kenroku.kanazawa-u.ac.jp
Bioscience, Biotechnology, and Biochemistry
|July 2, 2003
Abstract:
Myrothecium verrucaria bilirubin oxidase expressed in Aspergillus oryzae is in a resting form different from that of the authentic bilirubin oxidase, but reaches the resting form of the authentic enzyme after one cycle of reduction and reoxidation with dioxygen as shown by the absorption and electron paramagnetic resonance spectra.