Related Experiment Videos
Effects of mineral trioxide aggregate (MTA) extracts on mitogen-activated protein kinase activity in human
Tsui-Hsien Huang1, Shinn-Jyh Ding, Ting-Cheng Hsu
1Dental Department, Chung Shang Medical University Hospital, No. 110, Sec. 1, Chien-Kuo N. Road, Taichung, Taiwan.
Abstract:
Extracellular regulated kinases (ERKs)-1 and -2 are members of the MAPK family of protein kinases involved in the proliferation, differentiation and apoptosis of bone cells. The purpose of the present study investigated the biocompatibility role, and signaling pathways of components of mineral trioxide aggregate (MTA) by culturing human osteosarcoma cell line (U2OS) in the presence of materials. Biocompatibility effects were assessed using the MTT assay for mitochondrial enzyme activity. The statistical analysis of the survival rate was performed using one-way analysis of variance (ANOVA) with p<0.05 shown statistical difference. The signaling pathway of MTA-treated U2OS cells were assessed by the western blotting methods. Dose-dependent and time-dependent tests were conducted. The results showed that the survival rates of the MTA extract experimental groups were higher than that of the control group (p<0.05). ERKs activity was dose-dependent, decreasing as the concentrations of the MTA extract decreased, and was time-dependent, decreasing as the treatment time increased. Suppression of ERK pathway by PD98059 resulted in dose-dependent and time-dependent decreases. The findings suggest that MTA is a biocompatible material to U2OS cells, and the ERK kinase pathway plays a signal transduction role in the MTA treated U2OS cells.
Insights
Mineral trioxide aggregate (MTA) enhances human osteosarcoma cell survival, indicating biocompatibility. The extracellular regulated kinases (ERK) pathway is crucial for MTA
Area of Science:
- Biomaterials Science
- Cell Biology
- Molecular Biology
Background:
- Extracellular regulated kinases (ERKs)-1 and -2 are key MAPK family members regulating bone cell functions.
- Mineral trioxide aggregate (MTA) is widely used in dentistry, but its cellular effects require further elucidation.
Purpose of the Study:
- To investigate the biocompatibility of MTA components on human osteosarcoma cells (U2OS).
- To explore the role of signaling pathways, specifically ERK, in mediating MTA's effects on U2OS cells.
Main Methods:
- U2OS cells were cultured with MTA extracts.
- Biocompatibility was assessed using MTT assay for mitochondrial activity.
- Signaling pathways were analyzed via Western blotting and ERK pathway suppression with PD98059.
Main Results:
- MTA extract significantly increased U2OS cell survival rates compared to the control (p<0.05).
- ERK activity showed a dose- and time-dependent relationship with MTA extract concentration and treatment duration.
- ERK pathway suppression by PD98059 led to decreased cell survival.
Conclusions:
- MTA demonstrates biocompatibility with U2OS cells.
- The ERK kinase pathway is implicated in the signal transduction of MTA-treated U2OS cells.