Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Independently-addressable micron-sized biosensor elements.

C Brandon Davis1, Lisa M Shamansky, Steven Rosenwald

  • 1Department of Chemistry, University of California, Riverside, CA 92521, USA.

Biosensors & Bioelectronics
|July 2, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Discrete Protein-Surface Interactions in Scanning Force Microscopy Adhesion Force Measurements.

Langmuir : the ACS journal of surfaces and colloids·2014
Same author

Long-observation-window band-selective homonuclear decoupling: increased sensitivity and resolution in solid-state NMR spectroscopy of proteins.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2013
Same author

Is the WHO 90:10 prostate-specific antigen (PSA) first international reference standard really 90% α1-antichymotrypsin-bound PSA and 10% free PSA?

Clinical chemistry·2011
Same author

Rapid fabrication of a poly(dimethylsiloxane) microfluidic capillary gel electrophoresis system utilizing high precision machining.

Lab on a chip·2004
Same author

Microchip capillary gel electrophoresis with electrochemical detection for the analysis of known SNPs.

Lab on a chip·2004
Same author

Performance of pyrolyzed photoresist carbon films in a microchip capillary electrophoresis device with sinusoidal voltammetric detection.

Analytical chemistry·2003
Same journal

Integration of electrochemical sensors in organ-on-a-chip microfluidic platforms: Advances and perspectives.

Biosensors & bioelectronics·2026
Same journal

DNN-PURE: A deep neural network approach to paper-based urea sensing.

Biosensors & bioelectronics·2026
Same journal

Rationally architected MOF-derived Co<sub>3</sub>O<sub>4</sub>@NiMn-LDH hollow heterostructure-based sensor array empowering sensitive detection and discrimination of neurological biomarkers.

Biosensors & bioelectronics·2026
Same journal

Four-in-one multifunctional CoCu-NC@AuPt nanozyme integrated M13 phage-displayed nanobody based multimodal lateral flow immunoassay for bovine lactoferrin detection.

Biosensors & bioelectronics·2026
Same journal

A novel capillary-driven dual-mode imaging flow cytometry system for malaria parasite detection and quantification.

Biosensors & bioelectronics·2026
Same journal

Liver-targeted alkaline phosphatase-activatable fluorescent probe for imaging liver fibrosis and screening anti-fibrotic natural products.

Biosensors & bioelectronics·2026
See all related articles

Researchers developed a novel electrochemical method to attach DNA probes to gold surfaces. This technique enables precise immobilization for advanced biosensing applications.

Area of Science:

  • Biotechnology
  • Surface Chemistry
  • Molecular Biology

Background:

  • Micro-total analysis systems and biosensing require precise biomolecule immobilization.
  • Attaching biomolecules to specific surface areas is crucial for developing advanced analytical tools.

Purpose of the Study:

  • To develop a novel electrochemical method for spatially selective DNA probe immobilization on gold surfaces.
  • To demonstrate the specificity and efficiency of this attachment scheme for biosensing applications.

Main Methods:

  • Fabrication of micron-sized gold band electrodes on glass slides.
  • Electrochemical deposition of biotin-LC-hydrazide (BH) onto gold electrodes.
  • Immobilization of avidin and biotinylated DNA probes via a 'molecular sandwich' architecture.

Related Experiment Videos

  • Visualization of DNA immobilization and hybridization using fluorescence tags.

    • Main Results:

    • Successful electrochemical attachment of DNA probes to defined micron-sized regions of gold surfaces.
    • Demonstration of specific DNA probe attachment and hybridization with complementary DNA targets.
    • Spatially selective immobilization and hybridization of unique DNA sequences confirmed through fluorescence imaging.

    Conclusions:

    • The developed electrochemical method offers a precise and versatile approach for DNA probe immobilization.
    • This technique is suitable for creating high-density microarrays for biosensing and diagnostics.
    • The method's specificity ensures reliable detection of target DNA sequences.