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Updated: Sep 23, 2026

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
Published on: February 5, 2019
Good-Enough RFLP Matcher (GERM) program
Ian A Dickie1, Peter G Avis, David J McLaughlin
1Department of Forest Resources, University of Minnesota, 115 Green Hall, 1530 N Cleveland Ave.,St. Paul, MN 55108, USA. dicki017@umn.edu
Abstract:
A spreadsheet-based program (Good-Enough RFLP Matcher or GERM) is presented that matches unknown restriction fragment length polymorphism (RFLP) patterns of ectomycorrhizal fungi to a database of known ectomycorrhizal fungi. The program uses three simple methods to determine whether a sample matches a known: (1) Forward Matching: whether every band in the unknown is present in a known sample within a given error range; (2) Backward Matching: whether every band in the known sample is present in the unknown within a given error range; (3) Sum of Bands: whether the sum of all bands in the known and unknown are similar within a given error range. The program is available through the web page of this journal.
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