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Updated: Jul 29, 2026

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
Published on: December 9, 2017
Efforts toward creating unnatural base pairs for an expanded genetic code
I Hirao1, T Mitsui, T Fujiwara
1Yokoyama CytoLogic Project, ERATO, JST, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Abstract:
A series of unnatural base pairs was designed and examined for the expansion of the genetic alphabet and for a better understanding of the mechanism of nucleic acid biosyntheses. To improve the shape complementarity of the previously developed unnatural base pairs, 2-amino-6-(N,N-dimethylamino)purine (x)--pyridon-2-one (y) and 2-amino-6-(2-thienyl)purine (s)--y, the pyrimidine analogue, y, was replaced by a five-member ring, 4-imidazolin-2-one (z), and the s-z pairing in replication was examined. Unnatural bases based on the five-member ring were also applied to the development of non-hydrogen-bonded base pairs.
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