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Development of ribozyme synthesis system using a rolling-synchronization: effect of template DNA secondary structure
T Ohmichi1, M Nakamura, N Sugimoto
1High Technology Research Center, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Nucleic Acids Research. Supplement (2001)
|July 3, 2003
Abstract:
Circular DNA behaves as an efficient template for RNA polymerase (RNAP) in rolling circle transcription (RCT). We found that the short hairpin-stem structure in the circular DNA without promoter enhances the DNA recognition of T7 RNAP in RCT. The result indicates that T7 RNAP would distinguish the short DNA hairpin-stem structure.