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Preparations of a RNA-lipid complex film and its physical properties
Y Hoshino1, H Nakayama, Y Okahata
1Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuda, Midori-ku, Yokohama 226-8501, Japan.
Nucleic Acids Research. Supplement (2001)
|July 3, 2003
Abstract:
We extracted RNA (aver. 70-80 bases) from yeasts and prepared a RNA-lipid complex by replacing sodium counter cations with cationic amphiphiles (2C12N+2C1). The RNA-lipid complex was soluble only in organic solvents and formed a self-standing film by casting from organic solutions. The RNA film was physically strong, although its molecular weight is small and the RNA molecules are oriented in one direction by stretching the film, in which RNAs form intermolecular hydrogen bonds and show the physical strength of the film.