DNA hybridization using "bead-array": probe-attached beads arrayed in a capillary in a predetermined order

We present a DNA analysis device called "Bead-Array", in which DNA-probe-attached beads (100 microns) are arrayed in a capillary in a predetermined order. We developed this device to overcome the problems of using DNA microarray technology, including high cost and a lengthy analysis time. Bead-arrays can easily be mass-produced even for many different probe combinations, and the small reaction volume and the use of sample flows enables faster hybridization. To demonstrate this, we examined DNA hybridization experiments using 18-mer DNA probes and targets and compared them to ones with beads in tubes. The results show that hybridization in bead-arrays progresses more than 100 times faster and reach the plateau in less than three min. These features suggest that the bead-array particularly meets the need for high-speed and disposable devices, for example diagnosis devices.