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Classified fingerprinting: A method of comprehensive analysis for comparing megabase genomes
1Biosystems Research Department, Central Research Laboratory, Hitachi Ltd.
Nucleic Acids Research. Supplement (2001)
|July 3, 2003
Abstract:
The objective of the work we describe is to establish elementary methods for investigating the functions of genes; specifically, a fingerprinting method for analyzing entire DNA fragments in a mixture. Our goal is to develop a method for comparing genes with a size of several megabases. We improved a method of amplified fragment length polymorphism (AFLP) so that it could be used to analyze all the restriction fragments in a mixture. This method could be used to detect 90% of the DNA fragments produced from 100-kb model genomes by using a four-base cutter enzyme.