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Updated: Aug 9, 2026

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development
Published on: April 15, 2013
A novel detection method for 2'-deoxyoxanosine
M Yamada1, H Honjo, T Hasegawa
1Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan.
Abstract:
2'-Deoxyoxanosine (dOxo) is a novel DNA lesion produced from 2'-deoxyguanosine by the treatment with nitrous acid and nitric oxide. However, there has been no direct evidence on the formation of dOxo in vivo so far. In order to detect dOxo in vivo, we have explored a sensitive detection method specific for dOxo by using a fluorescent labeling reagent and HPLC analysis. An efficient method to detect the dOxo formation in DNA is described.
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