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Evidence for a Schiff base formation of peptides derived from RecA with single-stranded oligonucleotides containing
T Sugiyama1, A Kittaka, H Takayama
1Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Nucleic Acids Research. Supplement (2001)
|July 3, 2003
Abstract:
We report some evidence for a Schiff base formation of 5-formyl-2'-deoxyuridine containing oligonucleotides with oligopeptide derived from the DNA binding site of RecA protein. We have demonstrated that cross-linked products were successfully detected by lowering the peptide concentrations. This is probably due to the reduced formation of large aggregates.