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Template directed DNA photoligation via substituted 2'-deoxyuridine
K Fujimoto1, N Ogawa, S Matsuda
1Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8501, Japan.
Nucleic Acids Research. Supplement (2001)
|July 3, 2003
Abstract:
An efficient and reversible template-directed photoligation of oligodeoxynucleotides (ODNs) using 5-carboxyvinyldeoxyuridine (CVU) was described. We have also demonstrated about photochemical synthesis of circularized DNA and padlocking plasmid DNA via CVU mediated photoreaction.