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Design and properties of trans-acting HDV ribozymes with extended substrate recognition regions
1Department of Environment and Natural Sciences, Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.
Nucleic Acids Research. Supplement (2001)
|July 3, 2003
Abstract:
We constructed a new type of trans-acting HDV ribozyme which is based on the antigenomic RNA sequence and has an additional binding site to form an extra stem (P5) of 7 base-pairs introduced in the J1/2 region between P1 and P2 stems. A substrate RNA containing the two binding sequences was specifically cleaved while no selectivity was observed in the case of the wild-type ribozyme with only one binding site. Mutation to produce two mismatch base-pairs in the central part of the P5 stem abolished the specific cleavage.