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Isolation and characterization of three genes paralogous to mouse Ring3
Y Taniguchi1, H Suzuki, M Ohtsuka
1Department of Genetic Information, Division of Molecular Life Science, School of Medicine, Tokai University, Isehara, Kanagawa 259-1193, Japan.
Nucleic Acids Research. Supplement (2001)
|July 3, 2003
Summary
Four paralogous genes (RING3, ORFX, BRDT, HUNK1) share similar structures and functions. Their varied expression patterns in mice, including ubiquitous, testis-specific, and muscle-specific transcripts, highlight evolutionary diversification.
Area of Science:
- Genomics
- Molecular Biology
- Evolutionary Biology
Background:
- Syntenic chromosomal regions contain paralogous genes originating from ancestral gene duplications.
- The human RING3 gene exhibits paralogous relationships with ORFX, BRDT, and HUNK1 genes.
Purpose of the Study:
- To compare the structure and expression profiles of four paralogous genes: Ring3, Orfx, Brdt, and Hunk1 in mice.
- To investigate the evolutionary diversification of gene expression patterns.
Main Methods:
- Isolation of mouse Orfx, Brdt, and Hunk1 cDNA clones from mouse testis RNA.
- Comparison of protein structures, amino acid sequences, and expression patterns across various mouse tissues.
Main Results:
- All four encoded proteins share similar amino acid sequences, including two conserved bromodomains.
- Ring3, Orfx, and Hunk1 genes show ubiquitous expression in adult mouse tissues.
- Ring3, Orfx, and Brdt genes exhibit testis-specific transcription, while Hunk1 is specific to striated muscle.
Conclusions:
- The diversification of expression patterns among Ring3-related genes suggests evolutionary adaptation.
- Nucleotide variations in regulatory elements likely contribute to ubiquitous and tissue-specific gene expression during evolution.