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Related Experiment Videos

DP epitope mapping by using T-cell clones.

A Urlacher1, A Dormoy, M M Tongio

  • 1Histocompatibility Laboratory, Regional Center for Blood Transfusion, Strasbourg, France.

Human Immunology
|October 1, 1992
PubMed
Summary
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This study links human leukocyte antigen (HLA) class II DP DNA variations to cell surface expression. Researchers identified specific epitopes on the DP beta chain responsible for T-cell recognition, though some allospecificity remains unexplained.

Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Human Leukocyte Antigen (HLA) research

Background:

  • Human Leukocyte Antigen (HLA) class II DP molecules play a crucial role in immune responses.
  • Understanding the relationship between HLA DP DNA polymorphism and cell surface expression is vital for immunology.
  • Identifying specific HLA DP epitopes involved in alloreactivity is key to transplant compatibility and autoimmune disease research.

Purpose of the Study:

  • To investigate the correlation between genomic HLA class II DP DNA polymorphism and its cell surface expression.
  • To identify specific HLA DP epitopes responsible for T-cell mediated alloreactivity.
  • To elucidate the structural basis of HLA DP recognition by T-cells.

Main Methods:

  • Generation of anti-HLA DP T-cell clones against novel specificities.

Related Experiment Videos

  • Testing T-cell clones on Epstein-Barr virus immortalized B-lymphoblastoid cell lines (B-LCLs) and panel cells.
  • Oligotyping and DNA sequencing of HLA DP genes, specifically the second exon, to analyze polymorphism.
  • Comparison of DNA sequences with panel reactivity to define epitopes.

    • Main Results:

    • A correlation was established between DPa specificity and the DPB1*0402 allele.
    • HLA DPb specificity was resolved into DPB1*1001 and DPB1*1401.
    • Epitopes for DPB1*1001 and DPB1*1401 were defined and mapped to beta-chain residues involved in peptide binding.
    • DNA sequencing failed to explain the allospecificity of anti-DPa responses, suggesting involvement of other structural factors.

    Conclusions:

    • Genomic HLA class II DP DNA polymorphism directly influences cell surface expression and T-cell recognition.
    • Specific beta-chain residues within the HLA groove are critical for recognizing DPB1*1001 and DPB1*1401 epitopes.
    • The structural basis for anti-DPa allospecificity requires further investigation beyond DNA sequence analysis.