Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hair Cells01:22

Hair Cells

Hair cells are the sensory receptors of the auditory system—they transduce mechanical sound waves into electrical energy that the nervous system can understand. Hair cells are located in the organ of Corti within the cochlea of the inner ear, between the basilar and tectorial membranes. The actual sensory receptors are called inner hair cells. The outer hair cells serve other functions, such as sound amplification in the cochlea, and are not discussed in detail here.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Journal of Artificial Organs 2019: the year in review : Journal of Artificial Organs Editorial Committee.

Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs·2020
Same author

Journal of Artificial Organs 2018: the year in review : Journal of Artificial Organs Editorial Committee.

Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs·2019
Same author

Journal of Artificial Organs 2017: the year in review : Journal of Artificial Organs Editorial Committee.

Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs·2018
Same author

Journal of Artificial Organs 2016: the year in review : Journal of Artificial Organs Editorial Committee.

Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs·2017
Same author

Journal of Artificial Organs 2015: the year in review : Journal of Artificial Organs Editorial Committee.

Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs·2016
Same author

Journal of Artificial Organs 2014: the year in review.

Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs·2015

Related Experiment Video

Updated: Jul 13, 2026

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development
10:09

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development

Published on: June 16, 2022

In vitro keratin expression of hair cells.

Y Kitano1, K Saito, E Okamoto

  • 1Department of Dermatology, Hyogo College of Medicine, Japan.

The Journal of Dermatology
|November 1, 1992
PubMed
Summary

Researchers successfully cultured human hair follicle cells in vitro. These cells, identified by specific keratins, show potential for studying hair formation and related conditions.

More Related Videos

Whole Neonatal Cochlear Explants as an In vitro Model
07:12

Whole Neonatal Cochlear Explants as an In vitro Model

Published on: July 28, 2023

Isolation and Culture of Primary Cochlear Hair Cells from Neonatal Mice
06:07

Isolation and Culture of Primary Cochlear Hair Cells from Neonatal Mice

Published on: September 15, 2023

Related Experiment Videos

Last Updated: Jul 13, 2026

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development
10:09

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development

Published on: June 16, 2022

Whole Neonatal Cochlear Explants as an In vitro Model
07:12

Whole Neonatal Cochlear Explants as an In vitro Model

Published on: July 28, 2023

Isolation and Culture of Primary Cochlear Hair Cells from Neonatal Mice
06:07

Isolation and Culture of Primary Cochlear Hair Cells from Neonatal Mice

Published on: September 15, 2023

Area of Science:

  • Dermatology
  • Cell Biology
  • Hair Follicle Biology

Background:

  • Human hair follicle cells are crucial for hair growth.
  • Establishing reliable in vitro models is essential for research.

Purpose of the Study:

  • To isolate and culture human hair follicle cells.
  • To characterize the cultured cells for potential research applications.

Main Methods:

  • Human scalp hair follicles were isolated using enzymatic treatments (dispase, collagenase, trypsin).
  • Cells were cultured in MCDB 153 HAA medium with supplements and controlled calcium levels (0.1 mM).
  • Immunohistochemistry was used to identify cell-specific markers.

Main Results:

  • Isolated cells exhibited robust proliferation and could be subcultured 7-8 times.
  • Immunohistochemical analysis confirmed the presence of keratins specific to hair-forming cells.
  • The optimized culture medium supported cell viability and growth.

Conclusions:

  • A reproducible method for culturing human hair follicle cells was established.
  • These cultured cells retain key characteristics of hair-forming cells.
  • The established cell model offers a valuable tool for hair biology research.