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Non-radioactive hybridization in microwells using enzyme linked immune sorbent assay for detection of
1Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical University, Wuhan 430030.
Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao
|July 5, 2003
Abstract:
PCR enzyme linked immune sorbent assay (ELISA) was developed for detection of RT-PCR-amplified cytokeratin 19 (CK19) mRNA and carcinoembryonic antigen (CEA) mRNA. The non-radioactive hybridization was performed in a streptavidin-coated microwell with digoxigenin-labeled PCR products and with biotin-labeled capture probe. PCR ELISA was proved to be expedient, simple, sensitive and safe for identification of CK19-, CEA-RT-PCR products. These results were proven by sequencing.