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Updated: Jul 12, 2026

Enhanced Oil Recovery using a Combination of Biosurfactants
Published on: June 3, 2022
Production and characterization of biosurfactants from Bacillus licheniformis F2.2
Jiraporn Thaniyavarn1, Niran Roongsawang, Takayuki Kameyama
1Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Jiraporn.Th@Chula.ac.th
Abstract:
A biosurfactant-producing strain, Bacillus licheniformis F2.2, was isolated from a fermented food in Thailand. The strain was capable of producing a new biosurfactant, BL1193, as well as two kinds of popular lipopeptide biosurfactants, plipastatin and surfactin. Mass spectrometry and FT-IR analysis indicated that BL1193 had a molecular mass of 1,193 Da with no peptide portion in the molecule. While plipastatin and surfactin were abundantly produced in a nutrient YPD medium, BL1193 was produced only in a synthetic DF medium containing no amino acids. According to an oil displacement activity test, the specific activity of BL1193 (6.53 kBS units/mg) is equivalent to that of surfactin (5.78-6.83 kBS units/mg).
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