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Published on: April 8, 2015
Karyotype of cryopreserved bone marrow cells
M L L F Chauffaille1, R F Pinheiro, J T Stefano
1Disciplina de Hematologia e Hemoterapia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil. chauffaille@hemato.epm.br
Summary
Cryopreserved bone marrow cells offer a reliable alternative for chromosomal abnormality analysis in hematological disorders when fresh cells are unavailable. While freezing reduces cell viability and successful analysis rates, it preserves chromosome morphology.
Area of Science:
- Hematology
- Cytogenetics
- Oncology
Background:
- Chromosomal abnormalities are crucial for classifying hematological neoplastic disorders.
- Analyzing cryopreserved malignant cells is vital for retrospective studies.
- Standard cytogenetic analysis requires fresh bone marrow cells.
Purpose of the Study:
- To compare karyotype analysis between fresh and cryopreserved bone marrow cells.
- To evaluate the efficacy of granulocyte-macrophage colony-stimulating factor (GM-CSF) in stimulating mitosis in fresh and cryopreserved cells.
- To assess cell viability and chromosome morphology after cryopreservation.
Main Methods:
- Compared karyotypes of fresh and cryopreserved bone marrow cells from 25 patients with hematological disorders.
- Assessed cell viability before and after cryopreservation.
- Evaluated the effect of GM-CSF on mitotic index.
- Compared chromosome morphology between fresh and cryopreserved samples.
Main Results:
- Average cell viability decreased from 98.8% (fresh) to 78.5% (cryopreserved).
- Successful cytogenetic analysis rates were 76% for fresh cells versus 52% for cryopreserved cells.
- GM-CSF did not significantly affect the mitotic index.
- Chromosome morphology was subjectively similar in both fresh and cryopreserved cells.
Conclusions:
- Cryopreservation of bone marrow cells is a viable option for cytogenetic analysis when fresh samples are not feasible.
- Despite reduced success rates and viability, cryopreserved cells maintain chromosome morphology.
- Further research may optimize cryopreservation protocols to improve success rates.
