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Related Experiment Videos

Cyclin E overexpression and amplification in human tumours.

Peter Schraml1, Christoph Bucher, Heidi Bissig

  • 1Institute of Pathology, University Hospital, University of Basel, 4031 Basel, Switzerland. Peter.Schraml@unibas.ch

The Journal of Pathology
|July 8, 2003
PubMed
Summary

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Cyclin E gene amplification and protein accumulation were found in numerous human tumors. This study highlights cyclin E

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cyclin E amplification and overexpression are implicated in various cancers.
  • Previous studies were limited in scope, examining only specific tumor types for cyclin E alterations.
  • Determining the significance of potential oncogenes like cyclin E across diverse tumors was previously labor-intensive.

Purpose of the Study:

  • To comprehensively investigate cyclin E alterations (gene amplification and protein accumulation) across a wide spectrum of human tumor types.
  • To leverage tissue microarrays (TMAs) for efficient analysis of cyclin E in a large cohort of primary tumors and metastases.
  • To assess the prevalence and significance of cyclin E alterations in human tumorigenesis.

Main Methods:

  • Generation of extensive tissue microarrays (TMAs) comprising 3670 primary tumors from 128 types, 709 metastases, and 354 normal tissues.

Related Experiment Videos

  • Analysis of cyclin E gene amplification using fluorescence in situ hybridization (FISH).
  • Assessment of cyclin E protein accumulation via immunohistochemistry (IHC).

    • Main Results:

    • Cyclin E gene amplification was identified in 15 distinct tumor types and subtypes.
    • Cyclin E protein accumulation was observed in a broader range of 48 different tumor types.
    • The study successfully expanded the understanding of cyclin E alterations in human cancers through TMA technology.

    Conclusions:

    • The widespread occurrence of cyclin E gene amplification and protein overexpression across numerous tumor types suggests a significant role for cyclin E in tumor development.
    • Tissue microarray technology provides an efficient platform for large-scale molecular profiling of tumors.
    • Further research into cyclin E's oncogenic functions is warranted across diverse malignancies.