Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fast multi-dimensional Hadamard spectroscopy.

Eriks Kupce1, Ray Freeman

  • 1Varian, Inc, Eynsham, Oxford, UK. rf110@cam.ac.uk

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|July 11, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced Sensitivity and Resolution in Biomolecular CEST NMR Experiments Using the Extended Hadamard Encoding Scheme.

Analytical chemistry·2025
Same author

The NOAH HSQC-COSY module revisited: A theoretical and practical comparison of pulse sequences.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2024
Same author

Cross-Polarization Schemes for Improved Heteronuclear Transfers Involving Labile Protons in Biomolecular Solution NMR.

Angewandte Chemie (International ed. in English)·2023
Same author

A general scheme for generating NMR supersequences combining high- and low-sensitivity experiments.

Chemical communications (Cambridge, England)·2023
Same author

Uniform water-mediated saturation transfer: A sensitivity-improved alternative to WaterLOGSY.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2022
Same author

The Extended Hadamard Transform: Sensitivity-Enhanced NMR Experiments Among Labile and Non-Labile <sup>1</sup> Hs of SARS-CoV-2-derived RNAs.

Chemphyschem : a European journal of chemical physics and physical chemistry·2021
Same journal

Localization-driven exchange contrast in diffusion exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

4.5 Tesla superconducting miniature magnet in liquid nitrogen.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Folding and unfolding dynamics of a DNA aptamer studied by heteronuclear <sup>1</sup>H-<sup>13</sup>C correlation zz-exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Multi-spin control from one-spin pulses.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Altering MRI rotating frame relaxations by changing the truncation level of Hyperbolic Secant pulse.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Effects of proton exchange on the lifetimes of long-lived states in aliphatic chains.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
See all related articles

This study introduces a novel Hadamard mode for multi-dimensional Nuclear Magnetic Resonance (NMR) spectroscopy. This method significantly accelerates data acquisition without compromising sensitivity, enabling faster molecular structure determination.

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biophysical Chemistry

Background:

  • Multi-dimensional Nuclear Magnetic Resonance (NMR) spectroscopy is crucial for molecular structure elucidation.
  • Traditional NMR methods face limitations in data acquisition speed, hindering complex analyses.
  • Accelerating NMR experiments is vital for advancing fields like structural biology and drug discovery.

Purpose of the Study:

  • To develop a method for significantly accelerating multi-dimensional NMR experiments.
  • To demonstrate that speed enhancements can be achieved without sacrificing spectral sensitivity.
  • To provide a framework for converting existing NMR pulse sequences to a faster acquisition mode.

Main Methods:

  • Implementation of a frequency-domain multiplexing scheme to replace time-domain evolution.

Related Experiment Videos

  • Encoding excitation and transfer operations using Hadamard matrices.
  • Decoding detected NMR signals with reference to the same Hadamard matrices.
  • Conversion of traditional multi-dimensional NMR pulse sequences into the Hadamard mode.

    • Main Results:

    • Demonstrated significant speed-up factors in multi-dimensional NMR data acquisition.
    • Maintained or improved sensitivity per unit time compared to conventional methods.
    • Successfully applied the Hadamard mode to 3D TOCSY-HSQC experiments on strychnine at 700 MHz.
    • Achieved measurement time reductions of up to three orders of magnitude in specific cases.

    Conclusions:

    • The Hadamard mode offers a powerful strategy for accelerating multi-dimensional NMR spectroscopy.
    • This technique is broadly applicable, allowing conversion of most existing pulse sequences.
    • The substantial reduction in measurement time opens new possibilities for analyzing complex biological molecules and systems.
    • This advancement holds significant promise for structural biology, metabolomics, and drug discovery research.