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Effect of P-selectin monoclonal antibody on metastasis of gastric cancer and immune function
Jin-Lian Chen1, Wei-Xiong Chen, Jin-Shui Zhu
1Department of Gastroenterology, Shanghai Jiao-Tong University affiliated Sixth People's Hospital, Shanghai 200233, China. wqq_021002@163.com
Aim:
To investigate the effect of cell adhesion molecule P-selectin monoclonal antibody (Mab) on metastasis and immune function of mice orthototopically implanted with human gastric cancer tissue.
Methods:
SCID mice were implanted orthotopically with SGC-7901 human gastric carcinoma tissue. Starting from day 3 after operation, animals were given intravenously PBS or P-selectin Mab (100 microg/injection) (for both normal mice and tumor-implanted mice with tumors), twice weekly for 3 weeks. Two animals in each group were sacrificed randomly at the 1st, 2nd, 4th week and 6th week. While T cell and B cell transformation indices were determined with the (3)H TdR infiltration method, the NK cell activity was detected by the LDH release method.
Results:
The metastatic rate in the P-selectin Mab treated group was lower than that in the PBS treated group (with tumors). The NK activity of normal mice increased over time. The immune functions (T, B cell function, NK activity) of the tumor group in the 6th week were significantly lower than those in the 4th week, but the change was attenuated by P-selectin Mab.
Conclusion:
P-selectin Mab could suppress the metastasis of gastric cancer with no adverse effect on host immune function.
Insights
P-selectin monoclonal antibody (Mab) treatment reduced gastric cancer metastasis in mice. This P-selectin Mab intervention also helped maintain host immune function, showing no adverse effects.
Area of Science:
- Oncology
- Immunology
- Cell Biology
Background:
- Gastric cancer metastasis remains a significant challenge in treatment.
- P-selectin plays a role in cell adhesion and metastasis.
- Understanding the impact of P-selectin inhibition on immune function is crucial.
Purpose of the Study:
- To evaluate the effect of P-selectin monoclonal antibody (Mab) on gastric cancer metastasis.
- To assess the impact of P-selectin Mab on the immune function of tumor-bearing mice.
Main Methods:
- Orthotopic implantation of human gastric carcinoma (SGC-7901) in SCID mice.
- Intravenous administration of P-selectin Mab or PBS control.
- Assessment of metastasis, T cell, B cell, and NK cell activity over time.
Main Results:
- P-selectin Mab significantly reduced the metastatic rate compared to the control group.
- Tumor presence led to decreased immune function (T, B, NK cells) by week 6.
- P-selectin Mab treatment attenuated the decline in immune function in tumor-bearing mice.
Conclusions:
- P-selectin Mab effectively suppresses gastric cancer metastasis.
- P-selectin Mab demonstrates a protective effect on host immune function during cancer progression.
