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Updated: Jul 12, 2026
Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
Published on: July 8, 2012
Successful collection of peripheral blood stem cells from an infant with acute lymphoblastic leukemia using the
Insights
Collecting peripheral blood stem cells (PBSC) using the Haemonetics V50 is safe and painless for infants with acute lymphoblastic leukemia. This method, even in infants, allows for safe PBSC collection with minimal complications.
Area of Science:
- Pediatric Hematology
- Oncology
- Transplantation Immunology
Background:
- Acute lymphoblastic leukemia (ALL) is a common childhood cancer.
- Hematopoietic stem cell transplantation is a crucial treatment modality for ALL.
- Peripheral blood stem cells (PBSC) are increasingly used for stem cell collection.
Observation:
- A novel method for PBSC collection was employed in an 8-month-old infant with ALL.
- The collection utilized the Haemonetics V50 apheresis system via a single lumen Broviac catheter.
- The procedure was performed using an exchange transfusion method with a two-arm approach.
Findings:
- Successful collection of PBSC was achieved in the infant patient.
- The procedure was well-tolerated, with no significant adverse events reported.
- A notable observation was a reduction in platelet count by half post-procedure.
Implications:
- This technique demonstrates the feasibility and safety of PBSC collection in very young patients.
- The findings support the use of apheresis for stem cell collection in pediatric oncology.
- Painless and safe PBSC collection methods are vital for improving treatment outcomes in infants with leukemia.
Abstract:
Peripheral blood stem cells (PBSC) were collected using the Haemonetics V50 from an 8 month old infant weighing 7.8 kg suffering from acute lymphoblastic leukemia in the first complete remission. Leukapheresis was performed according to an exchange transfusion procedure by the two arm method using only a single lumen Broviac catheter. No problem occurred in the patient during this procedure except for a reduction (by half) of the initial platelet count. This method enables one to collect PBSC very safely, even from infants, in a manner that is painless for patients.
